Resuspension of DNA Sequencing Reaction Products in Agarose Increases Sequence Quality on an Automated Sequencer
نویسندگان
چکیده
منابع مشابه
Resuspension of DNA sequencing reaction products in agarose increases sequence quality on an automated sequencer.
We are investigating approaches to increase DNA sequencing quality. Since a majorfactor in sequence generation is the cost of reagents and sample preparations, we have developed and optimized methods to sequence directly plasmid DNA isolated from alkaline lysis preparations. These methods remove the costly PCR and post-sequencing purification steps but can result in low sequence quality when us...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2002
ISSN: 0736-6205,1940-9818
DOI: 10.2144/02333st03